Database : HANSEN Search on : DNA BACTERIANO [Subject descriptor] References found : 51 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19844 Author: Cole, Stewart T. Title: The Mycobacterium leprae genome projest. Source: Int. J. Lepr;66(4):589-591, Dec. 1998. . Conference: Present in: International Leprosy Congress, 15, Beijing, 07-12 Sept. 1998. Descriptors: Mycobacterium leprae/genet DNA Bacteriano/quim Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1998/pdf/v66n4/v66n4repcur03.pdf / en Location: BR191.1

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Id: 17278 Author: Kim, Se-Kom; Lee, Seong-Beom; Kang, Tae-Jin; Chae, Gue-Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA Bacteriano Resistência Bacteriana a Múltiplas Drogas Hansenostáticos Hanseníase Mycobacterium leprae Reação em Cadeia da Polimerase Polimorfismo (Genética) Polimorfismo Conformacional de Simples Fita Análise de Sequência de DNA Limits: Humano Coréia (geográfico) Mutação Puntual Location: BR191.1; 09178/s

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Id: 17277 Author: Mohammad, Hatta Title: Epidemiology of leprosy: molecular, biological, and immunological approach ..- Source: s.l; s.n; 2003. 10 p. tab. Abstract: Leprosy is an infectious disease for which humans are considered the only source of infection. The major hindrance in leprosy control and thus in reaching the elimination goal is that numerous leprosy cases remain undetected for a long time. Many of these patients are a continuous source of infection and, and hence perpetuate transmission. The goal of the World Health Organization (WHO) is to eliminate leprosy as a public problem by the year 2000; that is, to reach as a global prevalence of <1 per 10,000 people. The epidemiological data generated routinely by health services are greatly influenced by their policies and activities. The data do not, however necessarily reflect the true situation in the field. Information on the magnitude of the leprosy problem in any one area is important for the health services with regard to their planning, monitoring and evaluation of leprosy control activities. Our studies have suggested that the high prevalence of antibodies in children may be indicative of the active transmission of M. leprae in their surroundings. The prevalence of these antibodies may also be important for leprosy control programs in order to detect new patients as early as possible and in an effective and sustainable manner. Based on PCR data, it seems that the environment also plays an important role in the transmission of leprosy in endemic areas. The results of our study show that contact with a leprosy patient is the major determinant in the incidence of leprosy and that this concept shows similarities with the "stone-in-the-pond" principle of tuberculosis transmission in concentric circle around patients. (AU). Descriptors: Anticorpos Análise por Conglomerados DNA Bacteriano Predisposição Genética para Doença/*GE Geografia Incidência Hanseníase/BL/*EP/TM Mycobacterium leprae/GE/*PY Prevalência Estudos Soroepidemiológicos Limits: Humano Location: BR191.1; 09176/s

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Id: 13953 Author: Kim, Se -Kon; Lee, Seong- Beom; Kang, Tae -Jin; Chae, Gue -Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA BACTERIANO/quim DNA BACTERIANO/genet Resistência Bacteriana a Múltiplas Drogas/genet COREIA (GEOGRAFICO) HANSENOSTATICOS/farmacol HANSENIASE/quimioter HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MUTACAO PUNTUAL REACAO EM CADEIA DA POLIMERASE/métodos POLIMORFISMO (GENETICA) POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUÊNCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 09096/s

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Id: 13931 Author: Honore, N Title: Le genome de Mycobacterium leprae: de l'analyse de la sequence aux enjeux therapeutiques The Mycobacterium leprae genome: from sequence analysis to therapeutic implications- Source: s.l; s.n; 2002. 7 p. ilus, tab. Abstract: L'analyse génomique de Mycobacterium leprae, l'agent pathogène de la lèpre, a été réalisée grâce au séquençage à haut débit des cosmides et plasmides préparés à partir de l'ADN d´une souche isolée d'un patient. Elle a montré que ce bacille possède un seul chromosome circulaire, dont la taille (3,2Mb) et la composition en G+C (57,8%) sont réduits par rapport aux austres chromosomes mycobactériens connus. L'analyse informatique de la séquence a mis en évidence que seule la moitié de la séquences est codante. L'autre moitié contient des pseudogènes et des séquences non codantes. Le génome de M. leprae a donc subi une évolution réductive très importante qui ne lui a laissé qu'un set minimun de gènes fonctionnels pour vivre. Les informations déduites de la partie codante de la séquence permettent d'appréhender les propriétés biologiques particulières de cet agent infectieux à développement intra-cellulaire obligatoire. De plus, la disparition de nombreuses voies enzymatiques par rapport à M. tuberculosis, autre pathogène intra-cellulaire en certains points semblable à M. leprae, explique les différences observées entre les deux organismes. Enfin, l'analyse génomique du bacille lépreux permet de comprendre les bases moléculaires de sa résistance à différents antibiotiques et d'identifier des cibles potentielles pour la mise au point de nouveaux traitements. (AU). Descriptors: DNA BACTERIANO/anal CROMOSSOMOS BACTERIANOS/genet COSMIDIOS/genet HANSENIASE/quimioter HANSENIASE/fisiopatol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/fisiol MYCOBACTERIUM LEPRAE/patogen GENOMA PLASMIDEOS ANALISE DE SEQUÊNCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T RESUMO EM INGLES Location: BR191.1; 09108/s

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Id: 13229 Author: Hackel, C; Houard, S; Portaels, F;; Elsen, A. van; Herzog, A; Bollen, A Title: Specific identification of Mycobacterium leprae by the polymerase chain reaction ..- Source: s.l; s.n; jun. 1990. 6 p. ilus, tab. Abstract: Oligonucleotide primers have been used to amplify DNA regions of the M. leprae genome by the polymerase chain reaction. A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa antigen of M. leprae. A second pair of primers, targetted to the same gene, leads to the amplification of a 154 bp DNA piece conserved in mycobacteria. Primers PLp1 and PLp2 discriminate the pathogenic species from other mycobacteria, detect down to 40 bacilli, and constitute potentially useful tools for the identification of M. leprae in clinical specimens.(AU). Descriptors: AMPLIFICACAO DE GENES HANSENIASE/diag HANSENIASE/microbiol DNA BACTERIANO/genet DNA BACTERIANO/isol MYCOBACTERIUM/genet MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/imunol MYCOBACTERIUM LEPRAE/isol Limits: HUMANO ANIMAL SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 02123/s

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Id: 12248 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; Jun. 2001. 6 p. ilus, tab. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA Análise Heteroduplex/*MT Location: BR191.1; 08678/s; BR191.1; 08430/s

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Id: 12241 Author: Chae, Gue-Tae; Kim, Min-Joo; Kang, Tae-Jin; Lee, Seong-Beom; Shin, Hang-Kye; Kim, Jong-Pill; Ko, Young-Hoon; Kim, Sung-Hwa; Kim, Nan-Hee Title: DNA-PCR and RT-PCR for the 18-kDa gene of mycobacterium leprae to asses the efficacy of multi-drug therapy for leprosy ..- Source: s.l; s.n; 2002. 6 p. tab, graf. Descriptors: PROTEINAS DE BACTÉRIAS BIOPSIA DNA BACTERIANO DENSITOMETRIA QUIMIOTERAPIA COMBINADA ELETROFORESE EM GEL DE AGAR GLICOLIPIDIOS GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE ESTUDOS LONGITUDINAIS CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO REPRODUTIBILIDADE DE RESULTADOS Location: BR191.1; 08644/s

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Id: 12238 Author: Maeda, Shinji; Matsuoka, Masanori; Nakata, Noboru; Kai, Masanori; Maeda, Yumi; Hashimoto, Ken; Kimura, Hiroaki; Kobayashi, Kazuo; Kashiwabara, Yoshiko Title: Multidrug resistant mycobacterium leprae from patients with leprosy ..- Source: s.l; s.n; Dec. 2001. 5 p. tab, graf. Descriptors: AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA ANTIBIOTICOS ANTITUBERCULOSE ANTIBIOTICOS ANTITUBERCULOSE SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO RESISTENCIA MICROBIANA A DROGAS RESISTENCIA A MULTIPLAS DROGAS GENES BACTERIANOS GENES BACTERIANOS LEPROSTATICOS LEPROSTATICOS HANSENIASE HANSENIASE CAMUNDONGOS DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE OFLOXACINA OFLOXACINA RIFAMPINA RIFAMPINA REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA Limits: ANIMAL Location: BR191.1; 08640/s

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Id: 12229 Author: Santos, Adalberto Rezende; Balassiano, Vivian; Oliveira, Maria Leide W; Pereira, Marcia Aparecida da Silva; Santos, Patricia Barros; Degrave, Wim Maurits; Suffys, Philip Noel Title: Detection of mycobacterium leprae DNA by polymerase chain reaction in the blood of individuals, eight years after completion of anti-leprosy therapy ..- Source: Rio de Janeiro; s.n; Nov. 2001. 5 p. tab. Descriptors: DNA BACTERIANO LEPROSTATICOS HANSENIASE ESQUEMA DE MEDICAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE ESTUDOS RETROSPECTIVOS Limits: ESTUDO COMPARATIVO Location: BR191.1; 08712/s; BR191.1; 08725/s

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Id: 12223 Author: Kremer, Laurent; Gurcha, Sudagar S; Bifani, Pablo; Hitchen, Paul G; Baulards, Alain; Morris, Howard R; Dell, Anne; Brennan, Patrick J; Besra, Gurdyal S Title: Characterization of a putative alpha-mannosyltranferase involved in phosphatidylinositol trimannoside biosynthesis in mycobacterium tuberculosis ..- Source: s.l; s.n; 2002. 11 p. ilus, graf. Descriptors: SEQUENCIA DE AMINOACIDOS DNA BACTERIANO GUANOSINA DIFOSFATO MANOSE LIPOPOLISSACARIDIOS MANOSILTRANSFERASES MANOSILTRANSFERASES DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM BOVIS MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS FOSFATIDILINOSITOIS ALINHAMENTO DE SEQUENCIA ESPECTROMETRIA DE MASSA DE BOMBARDEAMENTO RAPIDO Location: BR191.1; 08706/s

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Id: 12123 Author: Cole, S. T; Eigimeier, K; Parkhill, J; James, K. D; Thomson, N. R; Wheeler, P. R; Honore, N; Garnier, T; Churcher, C; Harris, D; Mungall, K; Basham, D; Brown, D; Chillingworth, T; Connor, R; Davies, R. M; Devlin, K; Duthoy, S; Feltwell, T; Fraser, A; Hamlin, N; Holroyd, S; Hornsby, T; Jagels, K; Lacroix, C; Maclean, J; Moule, S; Murphy, L; Oliver, K; Quail, M. A; Rajandream, M.-A; Rutherford, K. M; Rutter, S; Seeger, K; Simon, S; Simmonds, M; Skelton, J; Squares, S; Stevens, K; Taylor, K; Whitehead, S; Woodward, J. R; Barrell, B. G Title: Massive gene decay in the leprosy bacillus ..- Source: s.l; s.n; feb. 2001. 5 p. ilus, tab, graf. Descriptors: TATUS DNA BACTERIANO METABOLISMO ENERGÉTICO EVOLUÇAO MOLECULAR GENOMA BACTERIANO HANSENIASE DADOS DE SEQUENCIA MOLECULAR GENES REITERADOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE ANALISE DE SEQUENCIA DE DNA Transferência Genética Horizontal Limits: ANIMAL Location: BR191.1; 08699/s

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Id: 11758 Author: Kremer, Laurent; Gurcha, Sudgar S; Bifani, Pablo; Hitchen, Paul G; Baulards, Alain; Morris, Howard R; Dell, Anne; Brennan, Patrick J; Besra, Grudyal S Title: Charaterization of a putative x-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in Mycobacterium tuberculosis ..- Source: s.l; s.n; 2002. 10 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS DNA BACTERIANO GUANOSINA DIFOSFATO MANOSE LIPOPOLISSACARIDIOS MANOSILTRANSFERASES MANOSILTRANSFERASES DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM BOVIS MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS FOSFATIDILINOSITOIS ALINHAMENTO DE SEQUENCIA ESPECTROMETRIA DE MASSA DE BOMBARDEAMENTO RAPIDO TECNICAS DE FISIOTERAPIA/ED/MT MYCOBACTERIUM SMEGMATIS/EN Location: BR191.1; 08510/s

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Id: 11757 Author: Chae, Gue-Tae; Kim, Min-Joo; Kang, Tae-Jin; Lee, Seong-Beom; Shin, Hang-Kye; Kim, Jong-Pill; Ko, Young-Hoon; Kim, Sung-Hwa; Kim, Nan-Hee Title: DNA-PCR and RT-PCR for the 18-KDa gene of Mycobacterium leprae to asses the efficacy of multi-drug therapy for leprosy ..- Source: s.l; s.n; 2002. 6 p. tab. Descriptors: PROTEINAS DE BACTÉRIAS BIOPSIA DNA BACTERIANO DENSITOMETRIA QUIMIOTERAPIA COMBINADA ELETROFORESE EM GEL DE AGAR GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE ESTUDOS LONGITUDINAIS CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO REPRODUTIBILIDADE DE RESULTADOS IMUNOGLOBULINA M/BL CAMUNDONGOS REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE CAMUNDONGOS NUS REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA Limits: ANIMAL Location: BR191.1; 08517/s

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Id: 11750 Author: Cambau, Emmanuelle; Bonnafous, Pascale; Perani, Evelyne; Sougkoff, Wladimir; Ji, Baohong; Jarlier, Vicent Title: Molecular detection of rifampin and ofloxacin resistance for patients Who experience relapse of multibacillary leprosy ..- Source: s.l; s.n; 2002. 7 p. tab. Descriptors: DNA BACTERIANO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE OFLOXACINA RECIDIVA RIFAMPINA ANTIBIOTICOS ANTITUBERCULOSE DNA TOPOISOMERASE (ATP-HIDROLISANTE) DNA TOPOISOMERASE (ATP-HIDROLISANTE) MODELOS ANIMAIS DE DOENÇAS TESTES DE SENSIBILIDADE MICROBIANA HANSENIASE MUTAÇAO PROTEINAS DE PLANTAS PROTEINAS DE PLANTAS Location: BR191.1; 08526/s; BR191.1; 08659/s

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Id: 11729 Author: Nishimura, Masayuki; Kwon, Kyung Sool; Shibuta, Kenji; Yoshikawa, Yasuji; Oh, Chang-Keun; Suzuki, Tomokazu; Chung, Tae-Ahn; Hori, Yoshiaki Title: An improved method for DNA diagnosis of leprosy using formaldehyde-fixed, paraffin-embedded skin biopsies ..- Source: s.l; s.n; 1994. 4 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES DNA BACTERIANO ELETROFORESE EM GEL DE AGAR FORMALDEIDO DADOS DE SEQUENCIA MOLECULAR INCLUSAO EM PARAFINA REAÇAO EM CADEIA POR POLIMERASE SEQUENCIAS REGULADORAS DE ACIDO NUCLÉICO SENSIBILIDADE E ESPECIFICIDADE PELE FIXAÇAO DE TECIDOS Location: BR191.1; 07218/s

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Id: 11690 Author: Shin, Yoo-Chul; Lee, Hyejon; Lee, Hyeyoung; Walsh, Gerald P; Kim, Joo-Deuk; Cho, Sang-Nae Title: Variable numbers of TTC repeats in Mycobacterium leprae DNA from leprosy patients and use in strain differentiation ..- Source: s.l; s.n; 2000. 4 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE AUTO-RADIOGRAFIA DNA BACTERIANO REAÇAO EM CADEIA POR POLIMERASE REPRODUTIBILIDADE DE RESULTADOS REPETIÇOES DE TRINUCLEOTIDIOS Location: BR191.1; 08482/s

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Id: 11682 Author: Ramasoota, Pongrama; Wongwit, Waranya; Sampunachot, Prasert; Unnarat, Komes; Ngamying, Maeya; Svenson, Stefan B Title: Multiple mutations in the rpoB gene of Mycobacterium leprae strains from leprosy patients in Thailand ..- Source: s.l; s.n; 2000. 5 p. tab. Descriptors: HANSENIASE HANSENIASE LEPROSTATICOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE CODON DNA BACTERIANO MUTAÇAO PROTEINAS DE PLANTAS REAÇAO EM CADEIA POR POLIMERASE RIFAMPINA ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 08474/s

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Id: 11607 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; 2001. 6 p. tab, graf. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA ANALISE HETERODUPLEX/*MT Location: BR191.1; 08430/s

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Id: 11594 Author: Martin, E; Roche, P. W; Triccas, J. A; Britton, W. J Title: DNA encoding a single mycobacterial antigen protects against leprosy infection ..- Source: s.l; s.n; 2001. 6 p. tab. Descriptors: ANTIGENOS DE BACTÉRIAS VACINA BCG VACINAS BACTERIANAS VACINAS BACTERIANAS VACINAS BACTERIANAS SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO INTERFERON TIPO II HANSENIASE HANSENIASE HANSENIASE CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE LINFOCITOS T VACINAS DE DNA/GE/IM/*PD Limits: ANIMAL Location: BR191.1; 08417/s

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